LOCUS       V002764                 3018 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002764
VERSION     V002764
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ohashi-Kunihiro S, Yohda M, Masaki H, Machida M.
  TITLE     A novel vector for positive selection of inserts harboring an open
            reading frame by translational coupling
  JOURNAL   BioTechniques 43 (6), 751-752 (2007)
   PUBMED   18251250
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Ohashi S, Machida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2007) Contact:Sumiko Ohashi National Institute of
            Advanced Industrial Science and Technology (AIST), Research
            Institute for Cell Engineering; Central 6, 1-1, Higashi, Tsukuba,
            Ibaraki 305-8566, Japan
REFERENCE   3  (bases 1 to 3018)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3018)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "BioTechniques"; date: "2007"; volume: "43"; issue: "6"; pages:
            "751-752"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JUL-2007) Contact:Sumiko Ohashi National Institute of Advanced
            Industrial Science and Technology (AIST), Research Institute for
            Cell Engineering; Central 6, 1-1, Higashi, Tsukuba, Ibaraki
            305-8566, Japan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(413..667)
                     /gene="imm"
                     /label="Colicin-E3 immunity protein"
                     /note="Colicin-E3 immunity protein from Escherichia coli.
                     Accession#: P02984"
     primer_bind     complement(797..813)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(821..837)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(845..875)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(890..911)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1199..1787)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1961..2818)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2819..2923)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgcggccgct caccaatcac
      421 catcacgata atcaaacgaa acaaaatact cattatcgga aatatcaatt tgatgattaa
      481 agtatggttg tagcaaaggt acccattcag ctataacatc aaagcaaccg ttattaacat
      541 tatccttaaa aggcacacct agactttcca taactgaacc gtcatctcca aaatcttttg
      601 aatactcctc acccttaaaa tcttctgtac ttttatcaaa ccaagttaaa tccaatttaa
      661 gtcccatcat tcattcaggc cgctttggcc agcgtagtct gggacgtcgt acttagttac
      721 ttacggccga ggcggcctcg gtacccgggg atcctctaga gtcgacctgc aggcatgcaa
      781 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      841 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      901 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      961 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1021 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1081 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1141 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1201 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1261 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1321 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1381 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1441 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1501 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1561 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1621 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1681 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1741 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1801 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1861 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1921 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1981 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2041 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2101 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2161 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2221 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2281 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2341 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2401 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2461 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2521 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2581 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2641 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2701 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2761 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2821 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2881 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2941 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3001 tcacgaggcc ctttcgtc
//
